FIELD: veterinary microbiology. SUBSTANCE: deep anaerobic cultivation of B. anthracis strain upon nutritive medium containing sodium bicarbonate should be carried out in fermenter by continuously aerating culture liquid either with gas or mixture of several gases (argon, helium, nitrogen, etc.). Moreover, native protective antigen could be obtained in fermenters by mixing and surface aeration of culture liquid (CL) with different gases or at bubbling method of CL mixing, only. Moreover, gas expenditure corresponds to 0.05-0.40 l/min/l CL. The method enables to increase anthracic antigen output by 2-4 times and decrease terms of its accumulation at cultivation stage up to 15-20 h. EFFECT: higher efficiency. 2 ex, 2 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PREPARING NATIVE SPORE SUSPENSION FOR ANTHRAX VACCINE PREPARING | 2001 |
|
RU2198921C2 |
| METHOD OF OBTAINING OF ANTHRACIC PROTECTIVE ANTIGEN | 2006 |
|
RU2340356C2 |
| METHOD OF PRODUCTION OF PROTECTIVE ANTIGEN AND PROTEIN OF S-LAYER EA1OF ASPOROGENOUS RECOMBINANT STRAIN IN anthracis 55 ΔTPA-1Spo | 2012 |
|
RU2492241C1 |
| METHOD FOR PREPARATION OF SEED CULTURE IN TECHNOLOGY OF ANTHRAX VACCINES | 2018 |
|
RU2694597C1 |
| ASPOROGENOUS RECOMBINANT STRAIN Bacillus anthracis 55ΔТПА-1Sро (pUB110РА-1) AS PRODUCER OF ANTHRAX MICROORGANISM ANTIGEN | 2006 |
|
RU2321629C1 |
| RECOMBINANT STRAIN Bacillus anthracis STIΔTPA-1 (pUB110PA-1) AS PRODUCER OF ANTHRAX MICROORGANISM ANTIGEN | 2006 |
|
RU2321628C1 |
| DNA-STRUCTURE ENCODING MODIFIED VERSION OF PROTECTIVE ANTIGENE BACILLUS ANTHRACIS | 2015 |
|
RU2622085C2 |
| METHOD TO PRODUCE ADJUVANT | 2012 |
|
RU2493257C1 |
| STRAIN BACILLUS ANTHRACIS KM89 AS PRODUCER OF ANTHRAX ANTIGENS | 2001 |
|
RU2180349C1 |
| STRAIN BACILLUS THURINGIENSIS - A PRODUCER OF ENDOTOXIN TO COLEOPTERUS INSECTS AND A METHOD OF ITS CULTURING | 1995 |
|
RU2108384C1 |
