FIELD: medicine; biotechnologies.
SUBSTANCE: plating of the vaccine strain on a sterile nutrient medium with sodium bicarbonate. Thus administer sodium bicarbonate into a nutrient medium throughout an exponential growth phase at 6-8 hours of the vaccine strain planting. After the planting process termination allocation, concentration and sterilisation of a protective antigen is performed on the equipment of ultra-and microstraining.
EFFECT: stable obtaining of a native anthracic protective antigen with the activity in "РДП" more that 100 EA·ml-1 and reduction of time and stages of reception of the sterile concentrated protective antigen with the activity in "РДП" more that 200 EA·ml-1.
3 tbl, 2 ex
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