FIELD: medicine; biotechnologies.
SUBSTANCE: plating of the vaccine strain on a sterile nutrient medium with sodium bicarbonate. Thus administer sodium bicarbonate into a nutrient medium throughout an exponential growth phase at 6-8 hours of the vaccine strain planting. After the planting process termination allocation, concentration and sterilisation of a protective antigen is performed on the equipment of ultra-and microstraining.
EFFECT: stable obtaining of a native anthracic protective antigen with the activity in "РДП" more that 100 EA·ml-1 and reduction of time and stages of reception of the sterile concentrated protective antigen with the activity in "РДП" more that 200 EA·ml-1.
3 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| DNA-STRUCTURE ENCODING MODIFIED VERSION OF PROTECTIVE ANTIGENE BACILLUS ANTHRACIS | 2015 |
|
RU2622085C2 |
| METHOD OF PRODUCTION OF PROTECTIVE ANTIGEN AND PROTEIN OF S-LAYER EA1OF ASPOROGENOUS RECOMBINANT STRAIN IN anthracis 55 ΔTPA-1Spo | 2012 |
|
RU2492241C1 |
| METHOD FOR OBTAINING ANTHRACIC NATIVE PROTECTIVE ANTIGEN | 2000 |
|
RU2223114C2 |
| METHOD FOR PREPARATION OF SEED CULTURE IN TECHNOLOGY OF ANTHRAX VACCINES | 2018 |
|
RU2694597C1 |
| COMBINED VACCINE AGAINST ANTHRAX IN ANIMALS | 2001 |
|
RU2220742C2 |
| METHOD FOR PREPARING CHOLERAGEN ANATOXIN | 2013 |
|
RU2535122C1 |
| METHOD TO OBTAIN DRY COMBINED ANTHRAX VACCINE | 1999 |
|
RU2181294C2 |
| METHOD OF CONCENTRATING SPORE CULTURE IN PRODUCTION OF ANTHRAX VACCINE PREPARATIONS | 1998 |
|
RU2151798C1 |
| METHOD FOR PREPARING CONCENTRATE OF BACTERIAL CELLS IN PRODUCTION OF ANTI-PLAGUE VACCINES | 2001 |
|
RU2215033C2 |
| METHOD OF PRODUCTION OF CONCENTRATE OF MICROBIAL CELLS FOR OBTAINING LIVE TULAREMIA VACCINE | 2013 |
|
RU2528878C1 |
