FIELD: biotechnology, molecular biology. SUBSTANCE: invention can be used for the development of functionally active tyrosine-specific protein kinase chimeras. The constructed chimeric receptor comprises b-noncellular moiety that recognize specifically cell-target or infectious agent-target, transmembrane domain and intracellular moiety representing human or swine protein-tyrosine kinase Syk, or protein-tyrosine kinase ZAP-70, or derivative of CD28. DNA encoding chimeric receptor is cloned into expressive cowpox virus vector that is used for transfection of mammalian cells. Transfected cells expressing two or three different chimeric receptors comprising, respectively, intracellular moiety of protein-tyrosne kinase Syk and CD28 or intracellular moiety of ZAP-70, Sre and CD28 are administrated to mammal in effective dose for destroying cell-target or infectious agent-target. Invention provides the development of agents that are able to target therapeutic cells for specific recognition and destroying cell-target or infectious agent-target. EFFECT: improved destroying method, valuable biological properties. 32 cl, 19 dwg
