METHOD FOR PREPARING IMMUNOGENIC PREPARATION FROM YERSINIA PESTIS EV Russian patent published in 2005 - IPC

FIELD: medicine, microbiology, immunology.

SUBSTANCE: method involves culturing cells of Yersinia pestis EV in solid nutrient medium up to the concentration 100 x 10⁹ cells/ml with physiological solution and treated with equal volume of 0.125% cetavlon solution for 48 h. After control for the specific sterility suspension is centrifuged at 6 000-8 000 rev/min at 4^oC for 20-30 min and supernatant is centrifuged at 16 000 rev/min at 4^oC for 20-30 min. Precipitate is washed out twice with physiological solution, distilled water and centrifuged again at 16 000 rev/min for 20-30 min after each washing out. Precipitate is resuspended in 0.1% sodium deoxycholate and shaken at room temperature for 10-20 min followed by treatment by ultrasonic oscillation at frequency 20 kHz by 3-4 cycles by 5-7 s in ice bath and centrifuged again at 16 000 rev/min at 4^oC for 30-40 min. Obtained precipitate is resuspended in distilled water, centrifuged at 16 000 rev/min for 30-40 min and precipitate is resuspended in minimal volume of distilled water and dried by lyophilization. Method is simple in performing and provides preparing the preparation with high immunogenicity. Invention can be used in manufacturing the chemical vaccine.

EFFECT: improved preparing method.

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