FIELD: biotechnology, biochemistry, enzymes.
SUBSTANCE: invention relates to methods for preparing biocatalyst based on immobilized enzymes. For preparing biocatalyst an insoluble porous material is added to polymer solution representing aminopolysaccharide with simultaneous addition protein and glutaraldehyde to the same solution followed by squeezing out the forming enzyme-containing composition and its successive treatment with sodium boron hydride and antibacterial substance solutions. In the process short reaction invention provides preparing biocatalyst with the concentration of immobilized protein up to 25.0 mg/g of catalyst and high catalytic activity up to 950 U/g of biocatalyst with respect to some organophosphorus compounds. Invention can be used for removing organophosphorus compounds from different surfaces, among them, from skin and the following detoxification, and for using as effective agents for individual protection also.
EFFECT: improved preparing method, valuable properties of biocatalyst.
3 cl, 9 dwg, 1 tbl, 6 ex
Title | Year | Author | Number |
---|---|---|---|
BIOCATALYST PREPARATION METHOD AND A BIOCATALYST FOR DETOXIFICATION OF ORGANOPHOSPHORUS NEUROTOXIC COMPOUNDS IN CONTINUOUS-FLOW SYSTEMS | 2006 |
|
RU2315103C1 |
BIOCATALYST AND METHOD FOR ITS PREPARING | 2002 |
|
RU2233327C2 |
METHOD FOR PRODUCING A HETEROGENEOUS BROMELINE PREPARATION WHICH IS COVALENTLY BOUND TO A CHITOSAN MATRIX | 2018 |
|
RU2711786C1 |
NANOSCALE ENZYME BIOCATALYST FOR in vivo DETOXIFICATION OF ORGANOPHOSPHOROUS COMPOUNDS | 2012 |
|
RU2525658C2 |
METHOD FOR OBTAINING HETEROGENIC BIOCATALYST BASED ON HYDROLASE OF ESTERS OF ALPHA AMINOACIDS, HETEROGENIC BIOCATALYST OBTAINED BY SUCH METHOD, AND SYNTHESIS METHOD OF AMINOBETA-LACTAM ANTIBIOTIC UNDER ACTION OF THIS HETEROGENIC BIOCATALYST | 2013 |
|
RU2535893C1 |
CRYO-FORMED BIOCATALYST ENZYME FOR ORGANOPHOSPHORUS COMPOUNDS DETOXIFICATION | 2015 |
|
RU2615176C1 |
METHOD FOR ENZYMATIC HYDROLYSIS OF ORGANOPHOSPHOROUS SOIL COMPOUNDS | 2011 |
|
RU2451077C1 |
IMMOBILISED BIOCATALYST FOR MICROBIOLOGICAL PRODUCTION OF PECTINASES | 2008 |
|
RU2383618C1 |
METHOD FOR OBTAINING IMMOBILISED BIOCATALYST FOR SYNTHESIS OF WATER SOLUTIONS OF AMIDES | 2011 |
|
RU2500814C2 |
METHOD OF OBTAINING A HETEROGENEOUS DRUG BASED ON COLLAGENASE AND CHITOSAN | 2017 |
|
RU2678435C2 |
Authors
Dates
2005-10-10—Published
2004-03-18—Filed