FIELD: medicine, embryology, in particular isolation of inner cell mass to produce human embryonic stem cell (hESC) lines.
SUBSTANCE: claimed method includes step of immobilization of embryo having pellucid zone, trophoblast and inner cell mass on blastocyst stage; providing of opening in embryo on blastocyst stage by using laser ablation; and removing of inner cell mass through said opening. Opening is crosses through pellucid zone and trophoblast. Laser ablation is carried out using contactless diode laser. Inner cell mass, isolated from embryo on blastocyst stage is useful in production of human embryonic stem cell lines.
EFFECT: simplified method for isolation of inner cell mass.
30 cl, 2 ex
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