METHOD FOR MAKING CHITOSAN-MELANIN COMPLEX OF DEAD BEES Russian patent published in 2010 - IPC C08B37/00 

FIELD: medicine.

SUBSTANCE: chitosan-melanin complex of dead bees can be used in veterinary science for making treatment-and-prophylactic preparations, in medicine for prevention and treatment of intestinal infectious diseases of various origin, and also wound healing ointments and as a radio protector of organism and skin. The method involves preliminary drying of chitin-melanin raw materials to humidity 4-6%, providing its chopping, then the dried mass is powdered, deproteinized with 10% alkali NaOH at temperature 78-82°C within 1.5-2.0 hours. Then 50% NaOH is added to the reaction mixture thereby reducing its concentration to 30% from the initial. The prepared mass is deacetylated at temperature 90-95°C within 1.5-2.0 hours, filtered. The filtered mass representing chitosan is prepared by washing the mass with distilled water to make pH=7. Melanin is precipitated from the solution remained after filtering by to concentrated hydrochloric acid to ensure changing pH of processed solution medium to sour area and flocculation representing melanin that is separated from a liquid phase by centrifugation, washed out; chitosan and melanin are dried separately at temperature 50-60°C to humidity of 8…10 % and mixed to prepare an end product of chitosan-melanin complex.

EFFECT: invention allows simplifying method with higher yield of chitosan and melanin.

1 dwg, 5 tbl, 5 ex