DEVICE AND METHOD FOR DETECTING FLOURESCENT MARKED BIOLOGICAL COMPONENTS Russian patent published in 2010 - IPC G01N33/49 G01N33/536 G01N33/58 G01N21/03 

FIELD: medicine.

SUBSTANCE: device comprises a measuring cavity for receiving and introducing a fluid sample. The measuring cavity has a set fixed thickness not exceeding 170 micrometres. The measuring cavity has a section fit for acquisition of its image. Within the measuring cavity, there is a dry reagent. The reagent contains as a component, a molecule conjugate with phosphor used for binding with biological components and with all other reacting components. The reacting components are soluble and/or suspended in the fluid sample. The method involves mixing of the reagent with the liquid sample to be introduced in the measuring cavity. A section of the sample in the measuring cavity is exposed to electromagnetic radiation of wavelength corresponding to wavelength of phosphor excitation. Phosphor marked biological components are detected through-thickness of the measuring cavity. Further, numerical analysis of the digital image follows to identify the biological components showing phosphor and to determine amounts of the biological components showing phosphor in the sample. The biological components are discernible on the digital image as fluorescing points emitting electromagnetic radiation of wavelength corresponding wavelength of phosphor emission.

EFFECT: device and method allow for higher effectiveness of numerical volume concentration of fluorescent marked biological components of the fluid sample.

24 cl, 4 dwg