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2 415 936

(13)

(51)

IPC

C12N15/09(2006-01-01)

C07K14/415(2006-01-01)

C12N1/13(2006-01-01)

(21) (22)

Application

2010100356/10, 2010-01-11

(24)

Start date

2010-01-11

(22)

Actual filing date

2010-01-11

(45)

Published

2011-04-10

(72)

Inventor

Filipenko Maksim LeonidovichKhrapov Evgenij Aleksandrovich

(73)

Holder

Uchrezhdenie Rossijskoj Akademii Nauk Institut Khimicheskoj Biologii I Fundamental'Noj Meditsiny Sibirskogo Otdelenija Ran So Ran)

METHOD FOR GREEN FLUORESCENT PROTEIN (GFP) SECRETION FROM PLANT CELLS Russian patent published in 2011 - IPC C12N15/09 C07K14/415 C12N1/13

Abstract RU 2415936 C1

FIELD: medicine.

SUBSTANCE: constructed are recombinant plasmid DNA pAUTL-GFP bearing a hybrid gene coding green fluorescent protein (GFP) and a signal sequence of autolysin from Chlamydomonas reinhardtii. The produced plasmid is used to transform an Agrobacterium strain. A suspension of the transformed agrobacterial cells in a logarithmic growth stage is incubated with cells of Chlorella microalgae for 14-16 hours A protein secretion level is evaluated by measuring fluorescence of a culture environment.

EFFECT: new compounds show effective biological properties.

2 cl, 1 dwg, 1 tbl, 2 ex

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RU 2 415 936 C1

Authors

Filipenko Maksim Leonidovich

Khrapov Evgenij Aleksandrovich

Dates

2011-04-10—Published

2010-01-11—Filed