METHOD FOR BLOOD CELL ANALYSIS FOR GLUCOSE CONTENT Russian patent published in 2011 - IPC G01N33/48 G01N33/49 

FIELD: medicine.

SUBSTANCE: patient's arterial whole blood is sampled. The blood sample is sounded in a dish with laser at long wave within the range 500-1100 nm spatially focused in a separate blood cell selected from erythrocytes or leukocytes. It is followed with measuring a Raman spectrum in the spectral range with Stockes frequency shift within 100 cm^-1 to 3200 cm^-1 from a set blood cell volume determined by a confocal volume described by an interfaced microdiaphragm diameter. The Raman spectrum of glucose, haemoglobin and water in the concentrations typical for their content in a blood cell are pre-measured. The Stockes spectral components are selected in the Raman spectrum to match the typical resonant oscillations of glucose molecules and to fall to a minimum of the Stockes spectral components of the Raman spectra of haemoglobin and water. The amplitude of the Stockes spectral components of glucose is measured and related to the amplitudes of the Stockes spectral components of a calibration curve of the Raman spectra of glucose generated in the known concentration of a glucose solution to measure the glucose content in a separate blood cell.

EFFECT: invention enables noninvasive glucose measurement inside a separate alive blood cell selected from erythrocytes or leukocytes.

5 dwg