MUTANT HEAT-LABILE ENTEROTOXIN E. coli Russian patent published in 2012 - IPC C07K14/245 A61K39/02 A61K38/16 C12N15/31 A61P31/04 

FIELD: chemistry.

SUBSTANCE: invention describes extracted polypeptide including mutant A subunit of heat-labile enterotoxin E.coli (LT) that has only one mutated residue which is K, R, H or Y compared to heat-labile enterotoxin of wild type containing particular amino-acid sequence (stated in description). Mutant A subunit contains amino-acid replacement in position that matches position 61 LT of the wild type. LT containing such mutant A subunit shows decreased toxicity compared to its wild type analog. According to the invention the polypeptide can be used as adjuvant. It also describes vaccine for immune response induction containing bacterial or viral antigen and polypeptide as an adjuvant. The invention describes nucleic acid coding mutant A subunit LT E.coli, expression vector and transformed cell containing such vector.

EFFECT: provides for production of vaccines using highly immunogenic and non-toxigenic adjuvant.

16 cl, 2 tbl, 7 ex