FIELD: medicine.
SUBSTANCE: invention aims at detecting mRNA expression of the p16(INK4a) gene in cervical tissue samples. A diagnostic technique for cervical intraepithelial neoplasms on the basis of detecting p16 gene mRNA in the cervical scraping material with the use of quantitative PCR involves sampling the examined cervical material, analysing p16 mRNA expression as a potential progression marker of cervical dysplastic processes, as well as analysing the mRNA concentration of hypoxanthine-guanine phosphoribosyltransferase (HPRT-1) gene. It is combined with evaluating the level of relative mRNA expression of the p16 gene to the level of mRNA expression of HPRT-1. The relation of the level of mRNA expression of the p16 gene to mRNA expression of HPRT-1 more than 93 relative units corresponds with squamous cell carcinoma.
EFFECT: technique enables higher effectiveness of diagnosing premalignant cervical processes.
1 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| DIFFERENTIAL DIAGNOSTIC TECHNIQUE FOR CERVICAL DYSPLASIA AND CERVICAL CANCER | 2012 |
|
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| METHOD FOR PREDICTION OF PROGRESSION OF LOW-GRADE CERVICAL SQUAMOUS INTRAEPITHELIAL LESION WITH UNDERLYING HUMAN PAPILLOMAVIRUS INFECTION | 2021 |
|
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| METHOD FOR DIFFERENTIAL DIAGNOSTICS OF CERVICAL INTRAEPITHELIAL NEOPLASIAS ASSOCIATED WITH HUMAN PAPILLOMA VIRUS OF HIGH ONCOGENIC RISK | 2020 |
|
RU2754494C1 |
| METHOD FOR ASSESSING DEGREE OF MALIGNANCY OF INTRAEPITHELIAL INVOLVEMENT OF SQUAMOUS EPITHELIUM OF UTERUS | 2023 |
|
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| METHOD FOR ASSESSING THE RISK OF PROGRESSION OF INTRAEPITHELIAL CERVICAL NEOPLASIA AND DEVELOPMENT OF CERVICAL CANCER BY DETERMINING THE LEVELS OF HUMAN MRNA GENES | 2015 |
|
RU2660360C2 |
| DIAGNOSTIC TECHNIQUE FOR HYPERPROLIFERATIVE CONDITIONS AND METHOD FOR ASSESSING RISK OF CERVICAL CANCER ACCOMPANYING CERVICAL INTRAEPITHELIAL NEOPLASIA AND PAPILLOMA VIRUS CARRIER STATE BY DETERMINIG MRNA OF FUNCTIONAL HUMAN GENE COMPLEX | 2012 |
|
RU2532344C2 |
| METHOD FOR CERVICAL CARCINOGENESIS DETECTION | 2015 |
|
RU2702334C2 |
| METHOD FOR PREDICTING THE RISK OF RECURRENCE IN HPV16-POSITIVE PATIENTS WITH SQUAMOUS INTRAEPITHELIAL HIGH-GRADE DISEASE ON THE BASIS OF DETERMINING THE PHYSICAL STATUS OF THE VIRUS | 2019 |
|
RU2719581C1 |
| METHOD OF PREDICTING PROGRESSION OF MILD INTRAEPITHELIAL NEOPLASIA IN WOMEN OF YOUNG AND FIRST PERIOD OF MATURE AGE | 2014 |
|
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| METHOD FOR EARLY DIAGNOSTICS OF CERVICAL INTRAEPITHELIAL NEOPLASIAS (CIN) BASED ON METABOLIC MARKERS STUDY | 2016 |
|
RU2629632C1 |
