FIELD: medicine.
SUBSTANCE: method for producing mesenchymal cells involves embryonal carcinoma cell growth in the presence of a differentiation-inducing factor specified in a group consisting of factors inducing neural crest cells, factors responsible for developing neural crest cells, and factors being growth promoters of neural crest cells, for formation of a cell population containing CD44-positive and CD29-positive cells and/or CD44-positive and CD106-positive cells after processing by the differentiation-inducing factor. After processing by the differentiation-inducing factor, CD44-positive and CD29-positive cells and/or CD44-positive and CD106-positive cells are selected from the cell population as mesenchymal cells for tooth formation. It results in producing mesenchymal cells for tooth formation which are induced from embryonal carcinoma cells and are CD44-positive and CD29-positive cells and/or CD44-positive and CD106-positive cells. Said cells are used in a method for tooth formation.
EFFECT: invention enables producing mesenchymal cells for tooth formation which are used for tooth regeneration.
9 cl, 18 dwg, 5 tbl, 7 ex
