METHOD OF DETERMINING ALCOHOLS IN BIOLOGICAL TISSUES AND EXCRETAS OF HUMAN BODY Russian patent published in 2014 - IPC G01N30/88 

FIELD: biotechnology.

SUBSTANCE: sample under study is placed in a sealed container of inert material, thermostated to the temperature above 25°C but below the destruction temperature of the biological object under study. From the thermostated sample the sampling of gas-vapour phase is carried out, which is examined by chromatography-mass spectrometry method by separation on the chromatographic column. Then the components of mixture of the gas-vapour phase are recorded as a series of chromatographic peaks in the chromatogram and are identified on time of their exit on the chromatogram and mass spectrum. Calculation of the concentration of alcohols is carried out in accordance with the obtained chromatogram according to the relevant peak areas of components of mixture of the gas-vapour phase.

EFFECT: increased sensitivity, accuracy and reliability of the identification and quantitative research, preservation of the object under study for possible repeat or additional studies.

1 tbl, 8 dwg