PRODUCTION OF HUMAN DAIRY OLIGOSACCHARIDES IN MICROBIAL PRODUCERS WITH ARTIFICIAL IMPORT/EXPORT Russian patent published in 2023 - IPC C12N15/67 C12N15/70 C12N9/10 C12P19/18 

FIELD: oligosaccharides.

SUBSTANCE: invention relates to methods for the production of oligosaccharides in genetically modified bacterial host cells, as well as genetically modified host cells used in the methods. The invention relates to the production of lacto-N-triose II by a genetically modified host microorganism cell. A method for producing lacto-N-triose II by a genetically modified host microorganism cell includes obtaining a genetically modified host microorganism cell that contains at least one recombinantβ -1,3-N-acetylglucosaminyltransferase, increased expression or activity of at least one sugar export protein capable of exporting lacto-N-triose II. The said cell is cultured and lacto-N-triose II is obtained from the medium. At the same time, at least one sugar exporting protein is selected from the group consisting of YjhB from Escherichia coli, ProP from Mannheimia succiniciproducens, SetA from Cedecea neteri, and functional fragments thereof. Also, a genetically modified host microorganism cell for the production of lacto-N-triose II is proposed.

EFFECT: improved export and production of an increased amount of lacto-N-triose II.

12 cl, 9 dwg, 3 tbl, 8 ex