FIELD: biotechnology.
SUBSTANCE: invention is a method of storing aspen plants under conditions in vitro, comprising culturing the microshoots of aspen on the nutrient medium WPM supplemented with sucrose of 10-20 g/l, agar-agar 9 g/l and vitamins MS of 1 ml/l, sorbitol 5-10 g/l, and mannitol 5-10 g/l. Storage of plants is carried out at a temperature of +4°C under conditions of light of 8 h during the day/16 h at night with the intensity of 2000 lux.
EFFECT: invention enables to improve the safety in vitro of cultures of valuable selective genotypes and genetically modified aspen clones.
2 cl, 1 dwg, 3 tbl
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