FIELD: medicine.
SUBSTANCE: invention refers to biotechnology, more specifically to detecting lung cancer by means of an aptamer and can be used in diagnostics. The aptamers are prepared by selection involving alternating rounds of positive selection of the aptamers to minced human tumour lung tissues sampled from oncological patients after the operation and of negative selection to healthy lung tissues and healthy whole blood to determine a pool of the highest-affinity aptamers to be cloned, sequenced and analysed for a binding specificity to tumour lung cells.
EFFECT: prepared aptamers possess high sensitivity to tumour debris and circulating tumour cells in peripheral blood of the patient suffering lung cancer that enables more effective diagnosis of human lung cancer.
3 cl, 2 dwg, 1 ex
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