METHOD FOR ASSESSING FUNCTIONAL STATE OF HUMAN LYMPHOCYTE Russian patent published in 2015 - IPC G01N33/49 G01N21/45 

FIELD: medicine.

SUBSTANCE: method involves interference microscopy in vitro of single peripheral lymphocytes; the first sample is recovered from a donor's blood cell suspension, microscoped in the interference microscope to image a mononuclear in the form of optical density areas in projections of separate organelles, and the following parameters are measured as follows: cytoplasmic index, phase thickness, area, equivalent diameters, phase volume, refractivity in the following lymphocyte organelles: an outer border of the periphery of the cytoplasm, a dense portion of the cytoplasm, a chondriome, a nucleus and a nucleolus; the second sample is recovered from the same donor's lymphocyte suspension, and after the lymphocyte suspension is exposed to an external factor, the lymphocytes are re-microscoped in the interference microscope; the above parameters of the above lymphocyte organelles are measured; that is followed by forming the second set of the phase thickness; the parameters of the first and second sets of the phase thickness are compared; the functional state of the human lymphocytes are assessed by correlation coefficients with specifying a percentage of probability.

EFFECT: higher prediction accuracy of the patient's immune response to the action of a pharmacological preparation, reduced probability of undesired side effects and time of analysis and cost of diagnosis.

2 cl, 2 tbl, 12 dwg