IN VITRO DIFFERENTIATION OF PLURIPOTENT STEM CELLS IN PANCREATIC ENDODERM CELLS (PEC) AND ENDOCRINE CELLS Russian patent published in 2018 - IPC C12N5/735

Abstract RU 2670064 C2

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of cellular biology and biotechnology, in particular to a method for producing chromogranins A(CHGA)-negative cells. Method comprises contacting the cells of a human definitive endodermal line (excluding cells that are derived from human embryonic stem cells obtained solely by a method requiring embryo destruction) in vitro with at least 50 ng/ml of a member of the TGF superfamily and at least 25 ng/ml member of the Wnt family.

EFFECT: invention enables obtaining chromogranin A(CHGA)-negative cells.

9 cl, 23 tbl, 70 dwg, 24 ex

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RU 2 670 064 C2

Authors

Agulnik Alan

D'Amur Kevin

Dates

2018-10-17—Published

2014-03-13—Filed