FIELD: biotechnology.
SUBSTANCE: invention relates to the field of cellular biology and biotechnology, in particular to a method for differentiating pluripotent stem cells, method for producing cells expressing markers indicative of a definitive endoderm, methods of differentiation of cells expressing markers indicating a definitive endoderm, method for producing cells expressing markers indicative of a pancreatic endoderm, method for producing cells expressing markers indicative of the endocrine part of the pancreas. Method includes sowing pluripotent stem cells that are not human embryonic stem cells onto a surface with a density of about 0.75⋅105 cells/cm2 up to about 2.0⋅105 cells/cm2, treating seeded pluripotent stem cells with a ROCK inhibitor, differentiating pluripotent stem cells into cells expressing markers indicating the definitive endoderm. Further, cells expressing markers indicating a definitive endoderm are differentiated into cells expressing markers indicating the pancreatic endoderm, or into cells expressing markers indicating the endocrine part of the pancreas.
EFFECT: invention makes it possible to improve the differentiation of pluripotent stem cells, which are not human embryonic stem cells.
38 cl, 6 dwg, 1 tbl, 2 ex
