FIELD: biotechnologies.
SUBSTANCE: invention discloses an encoding nucleotide sequence, first of all, a messenger RNA (mRNA) including or encoding a histone stem-loop-type structure and a poly(A)-sequence or a polyadenilination signal. Besides, it describes the application of a pharmaceutical composition, first of all, vaccine to be used for the application in the treatment of tumorous and cancer diseases, cardiovascular diseases, infectious diseases, etc. Additionally, it describes transcription in vitro, processes in vitro for increasing the protein expression level with the help of the nucleic acid that includes or encodes the stem-loop-type histone structure and poly(A)-sequence, or polyadenilation, and methods ex vivo and in vivo.
EFFECT: higher expression of the encoded protein.
14 cl, 23 dwg, 15 tbl, 11 ex
