ELECTROCHEMICAL METHOD FOR EXPRESS-ANALYSIS OF AMYLOID-BETA WITH METAL IONS Russian patent published in 2016 - IPC G01N27/00 

FIELD: chemistry.

SUBSTANCE: invention relates to analytical chemistry, electrochemistry and biochemistry, and concerns a method for rapid analysis of amyloid-beta with metal ions. Method comprises depositing on surface of printed graphite electrode an aliquot of solution of synthetic peptide amyloid-beta (1-16) in a buffer solution (reference). Other electrode is then coated with an equal aliquot of said peptide with investigated metal ions in ratio 1:1-1:1,000. After incubation for 10 minutes, electrochemical determination of amyloid-beta (1-16) in solution is performed on each electrode by recording square-wave current-voltage curve of oxidation of peptide. Method then includes measuring height and potential of obtained oxidation peak in range of 0.6-0.7 V (relative to a pseudo-silver chloride reference electrode) at neutral pH and change in intensity of signals and shift of potential maximum in area of more positive values relative to control, formation of complex and determining ratio of metal ions and amyloid-beta in obtained complex.

EFFECT: technical result is development of electrochemical express-analysis of amyloid-beta with metal ions.

1 cl, 3 dwg, 1 tbl