FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and is intended for non-invasive diagnosis of colorectal cancer. Method comprises sampling and transportation of samples of patient's stool, extraction of patient's stool total DNA and determining integrity of DNA by PCR-analysis. To assess quality and quantity of recovered DNA, PCR-analysis of two DNA fragments are carried out in duplex mode: first fragment length 141 CL., located in coding gene TR53, second fragment 153 CL., located in coding BLM gene region. To assess integrity of genomic DNA by PCR-analysis of first fragment is used with length of 800 CL., containing 7, 8 and 9 exons gene TP53, and second fragment length 2340 CL., containing 14 and 15 MLH1 gene exons. Detection of at least one of these two fragments in PCR products indicates presence of colorectal cancer in patient.
EFFECT: invention provides effective diagnosis of colorectal cancer at early stages.
5 cl, 1 ex
Title | Year | Author | Number |
---|---|---|---|
METHOD OF DETERMINING THE METHYLATION SITES PuCGPy REGULATORY REGIONS OF GENES-MARKERS OF COLORECTAL CANCER BY GLAD-PCR-ANALYSIS AND OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELLED PROBES FOR REALISING SAID METHOD | 2015 |
|
RU2596404C1 |
METHOD FOR DIAGNOSING METASTASES OF COLON CANCER | 2016 |
|
RU2647470C2 |
METHOD FOR DIAGNOSING COLORECTAL CANCER | 2021 |
|
RU2772203C1 |
METHOD OF DIFFERENTIATED USE OF DNA SEQUENCING DATA FOR PATIENTS AFTER KIDNEY TRANSPLANTATION | 2022 |
|
RU2803796C1 |
TEST SYSTEM “miR-M-SCREEN” FOR PREDICTING THE DEVELOPMENT OF METASTASES IN PATIENTS WITH COLORECTAL CANCER BASED ON THE LEVEL OF miR-26a AND miR-143 MICRO-RNA IN BLOOD PLASMA | 2022 |
|
RU2786386C1 |
SCREENING METHOD OF COLORECTAL CANCER DIAGNOSING | 2021 |
|
RU2798058C2 |
METHOD FOR DIAGNOSING COLORECTAL CANCER | 2023 |
|
RU2819181C1 |
METHOD FOR PREDICTION OF MALIGNANT TRANSFORMATION OF EROSIVE-ULCEROUS LICHEN ACUMINATUS OF ORAL MUCOSA | 2015 |
|
RU2580222C1 |
METHOD OF EARLY DIAGNOSIS OF RECTAL CANCER RELAPSES WHILE MONITORING COURSE OF DISEASE | 2023 |
|
RU2813653C1 |
METHOD OF DETERMINING PERSON'S PREDISPOSAITION TO DEVELOPMENT OF AGGRESSIVE NON-HODGKIN'S LYMPHOMAS | 2008 |
|
RU2373862C1 |
Authors
Dates
2016-06-10—Published
2015-04-27—Filed