MICROFLUIDIC SYSTEM FOR CONTROLLING CONCENTRATION OF MOLECULES FOR STIMULATING TARGET CELL Russian patent published in 2016 - IPC G01N33/50 B01L3/00 B81B1/00 G01N21/63 C12M3/06 

FIELD: measurement technology.

SUBSTANCE: invention relates to a microfluidic system and can be used for quantitative determination of response of living cells to certain molecules. Microfluidic system for controlling a card for concentration of molecules capable of stimulating target cells includes: microfluidic device (1); chamber (8) or additional microfluidic channel, having base (6), intended to receive target cell; microporous membrane (5), covering network of holes (47, 470); one or more means supply to supply one or each of microfluidic channels of fluid medium, wherein at least one of said fluids comprises stimulating molecules of target cell. Microfluidic device comprises n_c≥1 microfluidic channels equipped with at least one inlet hole (21, 22) for fluid and at least one outlet hole for fluid medium; as well as n₀≥2 holes (47, 470) in microfluidic channel (4, 40) or distributed over several microfluidic channels. Number n_c of microfluidic channels and n₀ holes are related by relationship $$n_0={\displaystyle {\sum }_{i=1}^{n_0}\raisebox{1ex}{$n_c$}\!\left/ \!\raisebox{-1ex}{$c_i$}\right.},$$ where 1<i<n_c, and n₀/c_i is number of holes per channel C_i.

EFFECT: higher accuracy of analysis and reducing duration of analysis.

15 cl, 12 dwg