HUMANIZED GNAWING ANIMALS THAT EXPRESS HEAVY CHAINS CONTAINING V DOMAINS Russian patent published in 2018 - IPC A01K67/27 C07K16/46 

FIELD: biochemistry.

SUBSTANCE: invention relates to the field of biochemistry, in particular to a mouse for the production of human immunoglobulin variable region sequences comprising an insert of one or more human gene segments V_L and one or more human gene segments J_L, the insertion of one or more human gene segments V_L and one or more human gene segments J_L and a nucleic acid sequence that encodes an ADAM6 protein to its cell and a hybridoma formed from said cell. Also disclosed is a method for producing an antigen-binding protein that binds to an antigen of interest using the above mouse. Invention also relates to a method for modifying a mouse immunoglobulin heavy chain locus for carrying out a mouse modification that reduces or eliminates the endogenous activity of ADAM6 in a male mouse, and performing a modification that includes inserting a nucleic acid sequence that encodes an ADAM6 protein into the mouse genome.

EFFECT: invention makes it possible to efficiently obtain an antigen-binding protein.

16 cl, 9 dwg, 1 tbl, 4 ex