FIELD: biotechnology.
SUBSTANCE: calibration panel of control positive samples with the concentrations of foot and mouth disease virus RNA is prepared. Foot and mouth disease virus RNA is isolated from the vaccine raw material. The eluates are added to the mixture of components for RT-PCR-PB. RT-PCR-PB is conducted, followed by detection of amplification products using a fluorescent PCR detector. The magnetude of the amplification threshold cycle is determined. The relationship between the threshold amplification cycle and the concentration of the 146S component of the foot and mouth disease virus in the raw material is determined.
EFFECT: invention allows rapid and highly-reliable determination of foot-and-mouth disease 146S component concentration in vaccine raw materials based on RT-PCR-PB, followed by application of the developed negative regression model.
4 cl, 1 dwg, 7 tbl, 6 ex
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