FIELD: biotechnology.
SUBSTANCE: group of inventions is described, including a probe, a method for detecting or quantifying a target nucleic acid molecule in a sample using a polymerase chain reaction (PCR) and a kit for a polymerase chain reaction (options). In one embodiment, the probe comprises a dye and quencher conjugation product linked via an oligonucleotide linker connecting the dye and quencher, wherein the dye is covalently attached to the first nucleotide of the oligonucleotide and the quencher is covalently attached to a second nucleotide of the oligonucleotide that differs from the first one.
EFFECT: invention expands the arsenal of means for the polymerase chain reaction.
20 cl, 1 ex
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