FIELD: biotechnology.
SUBSTANCE: described is a group comprising a nanoparticle for increasing the available surface area of a substrate for seeding and clustering in sequencing by synthesis (SBS) (embodiments) and a method for increasing seed density and monoclonal clustering in SBS (embodiments). In one embodiment, the nanoparticle comprises a framework, one template site for binding the template polynucleotide to the framework and a plurality of auxiliary sites for binding auxiliary oligonucleotides to the framework.
EFFECT: invention extends the range of means for increasing the available surface area of the substrate for seeding and clustering in sequencing by synthesis (SBS).
46 cl, 13 dwg, 1 tbl, 1 ex
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