METHOD FOR POLYELECTROLITE MICROCAPSULES PREPARATION Russian patent published in 2018 - IPC A61K9/50 A61K38/02 A61K47/36 

FIELD: biotechnology.

SUBSTANCE: solutions of polycation-carrageenan with a concentration of 8.0-12.0 mg/ml in a buffer solution with pH of 4.7-4.8, peptides with a concentration of 3.5-4.5 mg/ml and polyanion chitosan with a concentration of 8.0-12.0 mg/ml in a buffer solution with pH of 4.7-4.8 are prepared and mixed originally in the ratio of 1:5:1, followed by consecutive addition of polyelectrolyte solutions in the ratio of 1:1 to achieve a lasting polyelectrolyte complex, which is stirred for 30 minutes, centrifuged at 400-500 rpm for 10-15 minutes, followed by supernatant removal, sediment is washed with 0.5 M sodium chloride solution and the microcapsules obtained are stored in the form of suspension or freeze-dried at plus 2-4°C.

EFFECT: preparation of polyelectrolyte microcapsules that allow to deliver peptides from the embryonic tissues of birds to the intestines, bypassing the gastric environment, while maintaining their biological activity.

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