FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology, namely to improvement of the cell banks defrosting, freezing of cells of the Chinese hamster ovary (CHO), a composition for freezing CHO cells and a cell bank. Method involves freezing CHO cells in a freezing medium which contains a buffer solution and DMSO, at the pH of the freezing medium, brought to 7.5 to 8.5 before freezing.
EFFECT: invention allows increasing vitality and growth rate of cells during defrosting.
69 cl, 6 dwg, 1 ex
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Authors
Dates
2020-02-19—Published
2015-07-09—Filed