METHOD OF THE MELANOMA B MALIGNANT GROWTH IN MICE MODIFICATION WITH CHRONIC PAIN Russian patent published in 2018 - IPC G09B23/28 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to experimental studies in oncology, and can be used in the reproduction of melanoma B₁₆ aggressive atypical growth in mice. Method involves the melanoma malignant growth modification with chronic pain. For this purpose, 2 weeks before the tumor is transplanted, creating a chronic pain model by tying the sciatic nerves from two sides. Subsequently, subcutaneous implantation of a suspension of tumor cells of murine melanoma B₁₆ is carried out in a physiological solution in a volume of 0.5 ml in a dilution of 1:10. Evaluation of the experimental results is carried out in 1–4 weeks after the tumor is transplanted. Method provides a change in the melanoma B₁₆ pathogenesis in mice, leading to an early emergence of subcutaneous tumors, early metastasis, metastasis into atypical sites, and to early death of animals.

EFFECT: such an economical and affordable model can be used to find ways of malignant tumors with extensive metastasis drug treatment.

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