HIGH RESOLUTION FLUORESCENT MICROSCOPY WITH THE STRUCTURED EXCITATION BEAM Russian patent published in 2018 - IPC G01N21/64 

FIELD: measurement; test.

SUBSTANCE: substance single molecules are in the fluorescent state, in which they can be excited by excitation light for the fluorescence light emission. Distance between the substance single molecules in the sample region of interest is maintained at the minimum value of where λ is excitation light wavelength, n is the optical material refraction index, α is half of the optical system span angle, which directs the excitation light onto the sample, I is the excitation light in the sample maximum intensity, I_s is the depending on the substance excitation light intensity, with the fluorescence saturation. Substance single molecules are excited by excitation light for the fluorescence light emission. Excitation light intensity distribution comprises at least one zero point. Excited substance single molecules fluorescence light is recorded in different positions (x_N) by at least of the excitation light intensity distribution one zero point in the sample region of interest. Distances between the nearest neighboring positions (x_N), by at least of the excitation light intensity distribution one zero point, in which the substance excited single molecules fluorescence light is recorded, makes not more than half of the minimum value d. Locations (x_M) of the substance single molecules are derived from the corresponding molecule fluorescence light intensity (I) curve from positions (x_N) of at least one of the excitation light intensity distribution zero point in the sample region of interest.

EFFECT: invention relates to the substance single molecules in the sample location determination methods.

25 cl, 11 dwg