FIELD: biochemistry.
SUBSTANCE: invention relates to biochemistry, in particular double-stranded RNA (dsRNA) to suppress the expression of the target gene in Leptinotarsa or Lygus. Also disclosed are a DNA construct encoding said dsRNA, a prokaryotic host cell, a yeast or plant host cell containing said DNA construct, a composition comprising dsRNA, a transgenic plant modified with said dsRNA, and a seed obtained from said transgenic plant. Also disclosed are the use of said composition for controlling infection, a method for inhibiting the expression of a target gene with said dsRNA, a method for increasing crop yield of a plant, a method of creating a transgenic plant using said DNA construct, the use of said dsRNA for preventing or combating infection.
EFFECT: invention makes it possible to fight or prevent Leptinotarsa or Lygus pest infestation.
28 cl, 10 dwg, 15 tbl, 5 ex
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