FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. Invention is a cell culture medium containing tyrosine in concentration of 4 to 50 mM and polyvinyl alcohol (PVA) in concentration of 0.5 to 10 g/l and a cell culturing method involving bringing mammalian cells into contact with a cell culture medium human embryonic kidney (293), newborn hamster kidney (BHK) cells, Chinese hamster ovary (CHO) cells, Sertoli mice cells, African green monkey kidney cells (VERO-76), human cervical cancer cells (HeLa), dog kidney cells, Buffalo rat liver cells, human lung cells, human liver cells, mouse mammary tumor cells, TRI cells, MRC 5 cells, FS4 cells or human hepatoma line (Hep G2).
EFFECT: thus, improved medium for cultivation of cells with increased solubility of tyrosine for cultivation of cells of mammals in high density and for optimal production of proteins.
28 cl, 4 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| ADDING IRON TO IMPROVE CELL CULTURE | 2012 |
|
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| RATIONALLY DEVELOPED MEDIA FOR CELL CULTIVATION | 2007 |
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| METHOD OF PRODUCING POLYPEPTIDES | 2005 |
|
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| OBTAINING HETEROMULTIMERIC PROTEINS USING MAMMALIAN CELLS | 2015 |
|
RU2727012C2 |
| IMPROVED METHODS OF PRODUCING RECOMBINANT POLYPEPTIDE | 2014 |
|
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| NOVEL CONSTANT LINE OF HUMAN CELLS | 2010 |
|
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| PRODUCING GLYCOPROTEINS | 2007 |
|
RU2463345C2 |
| AGONIST NOTCH3 ANTIBODIES AND APPLICATION THEREOF FOR TREATING NOTCH3-ASSOCIATED DISEASES | 2007 |
|
RU2461569C2 |
| METHOD OF PRODUCING POLYPEPTIDES | 2015 |
|
RU2699715C2 |
