FIELD: medicine.
SUBSTANCE: proposed group of inventions relates to medicine. Proposed are a method for detecting a modified base in a double-stranded nucleic acid molecule and a method for identifying at least one compound capable of preventing the interaction of a protein with its binding site. At first, the double-stranded nucleic acid molecule is denatured, then it is renatured in the presence of a protein capable of binding to the modified base. Next, blockage of the renaturation of the double-stranded nucleic acid is detected and the position of said blockage in the double-stranded nucleic acid molecule is determined.
EFFECT: proposed group of inventions provides for the identification of various DNA modifications and the determination of DNA-protein binding by determining the position at which blockage occurs.
14 cl, 13 dwg
| Title |
Year |
Author |
Number |
| NUCLEASE-MEDIATED DNA ASSEMBLY |
2015 |
- Schoenherr Chris
- Mcwhirter John
- Momont Corey
- Macdonald Lynn
- Murphy Andrew J.
- Warshaw Gregg S.
- Rojas Jose F.
- Lai Ka-Man Venus
- Valenzuela David M.
- Montagna Caitlin
|
RU2707911C2 |
| METHOD OF IDENTIFICATION OF LIGANDS, METHOD OF ANTIBODY PREPARING, METHOD OF SELECTION OF NUCLEIC ACID, METHOD OF NUCLEIC ACIDS MIXTURE PREPARING, MIXTURE OF NUCLEIC ACIDS, NUCLEIC ACID LIGAND NO OCCURRING IN NATURE |
1991 |
|
RU2198931C2 |
| TREATMENT OF DISEASES CONNECTED WITH SITE-1 MEMBRANE-IMPACTED PEPTIDASE OF TRANSCRIPTION FACTORS (MBTPS1), BY INHIBITING NATURAL ANTISENSE TRANSCRIPT TO MBTPS1 |
2010 |
- Kollard Dzhozef
- Khorkova Sherman Olga
|
RU2639550C2 |
| TREATMENT OF TUMOR PROTEIN 63 (p63) RELATED DISEASES BY INHIBITION OF NATURAL ANTISENSE TRANSCRIPT TO p63 |
2010 |
- Kollard Dzhozef
- Khorkova Sherman Olga
|
RU2611186C2 |
| TREATMENT DISEASES, ASSOCIATED WITH INTERFERON-REGULATORY FACTOR 8 (IRF8), BY INHIBITION OF NATURAL ANTISENSE TRANSCRIPT TO IRF8 |
2011 |
- Kollard Dzhozef
- Khorkova Sherman Olga
|
RU2611187C2 |
| TREATMENT OF DISEASES ASSOCIATED WITH UNCOUPLING PROTEINS 2 (UCP2), BY INHIBITING OF NATURAL ANTISENSE TRANSCRIPT TO UCP2 |
2010 |
- Kollard Dzhozef
- Khorkova Sherman Olga
|
RU2619185C2 |
| TREATMENT OF RNase H1 RELATED DISEASES BY INHIBITION OF NATURAL ANTISENSE TRANSCRIPT TO RNase H1 |
2011 |
- Kollard Dzhozef
- Khorkova Sherman Olga
|
RU2611192C2 |
| TREATMENT OF DISEASES, ASSOCIATED WITH SEX HORMONES BINDING GLOBULIN (SHBG), BY INHIBITION OF NATURAL ANTISENSE TRANSCRIPT TO SHBG |
2011 |
- Kollard Dzhozef
- Khorkova Sherman Olga
|
RU2611191C2 |
| COMPOSITIONS AND METHODS WITH USE OF NUCLEIC ACIDS TARGETED AT NUCLEIC ACIDS |
2014 |
- Mej Endryu Pol
- Orvitts Rejchel I.
- Dudna Dzhennifer A.
- Berger Dzhejms M.
- Karter Mettyu Merrill
- Donokhyu Pol
|
RU2662932C2 |
| TREATMENT OF FIBROBLAST GROWTH FACTOR 21 (FGF21) RELATED DISEASES BY INHIBITION OF NATURAL ANTISENSE TRANSCRIPT TO FGF21 |
2011 |
- Kollard Dzhozef
- Khorkova Sherman Olga
|
RU2610661C2 |
