METHOD FOR ISOLATION AND ANALYSIS OF EXOSOMES Russian patent published in 2023 - IPC G01N33/50 

FIELD: cell biology.

SUBSTANCE: invention relates to cell biology and medicine, in particular to a method for isolating extracellular exosomes from animal and human biological fluids, as well as identifying molecules on their surface. To implement this method, a sample of biological fluid is first obtained. Then modified submicron particles are produced, having affinity for at least one marker and containing a core of nanoparticles in an inorganic matrix of silicon dioxide, coated. Further, the specified sample is contacted with modified submicron particles with the formation of particle-exosome complexes. After that, the particle-exosome complexes are separated from unbound exosomes and modified submicron particles. The specified submicron particle contains iron oxide nanoparticles in the core, coated with streptavidin and a polyelectrolyte shell formed by polyalylaminohyrochloride and polyacrylic acid, as well as guide aptamers immobilized on the surface of submicron particles and having affinity for at least one marker present in the extracellular exosome. The separation of the particle-exosome complex is carried out by a magnetic field gradient, and the size of the nucleus of submicron particles is selected with a diameter from 300 nm to 500 nm, the particle-exosome complex contains a darpin with a fluorescent label specific to the EpCAM membrane protein, immobilized on the surface of the exosomes, and guiding molecules specific to the CD63 membrane protein are used as an aptamer.

EFFECT: present invention enables to expand the methods for isolating and analyzing circulating exosomes and exosomal components in order to create new methods for diagnosing and monitoring oncological diseases. 2 cl, 5 dwg, 1 ex