FIELD: biotechnology.
SUBSTANCE: disclosed is a diagnostic technique for breast cancer and ovarian cancer. Method involves detecting the micro-RNA contained in the blood of a patient with oncological breast cancer and ovarian cancer NP-chip. NP-chip surface is covalently immobilized with a set of mRNA-associated mammary cancer and ovarian cancer o-DNA probes, NP-chip is incubated with immobilized on its surface o-DNA set in a sample containing microorganisms recovered from patient's blood, measuring change of value flowing through current NP-chip. Change occurs when complexes are formed between immobilized o-DNA probes and micro-RNA specific to said probes.
EFFECT: invention provides early diagnosis of breast cancer and ovarian cancer with high sensitivity of 10-17 M in real time without marks for several minutes.
1 cl, 2 dwg, 4 tbl, 3 ex
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Authors
Dates
2019-07-31—Published
2017-12-27—Filed