FIELD: genetic engineering; biotechnology.
SUBSTANCE: recombinant plasmid DNA pFUSE 1-68 MAR TSH with SEQ ID NO: 1, coding cDNA genes of alpha and beta subunits of recombinant thyrotropin alpha, and a cell line of Chinese hamster ovary rhTSHCHO cells producing recombinant human thyrotropin alpha and created by transformation of the CHO DG44 line by nucleofection of said recombinant plasmid DNA.
EFFECT: invention enables to obtain a highly productive stable producer line of recombinant human thyrotropin alpha, as well as synthesise both subunits of thyrotropin alpha, which reduces heterogeneity of the pool of transformed cells.
2 cl, 3 dwg, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT PLASMID DNA pBiIPr-ABIgA1FI6-INTHT FOR OBTAINING RECOMBINANT IGA1 ISOTYPE IMMUNOGLOBULIN | 2016 |
|
RU2664184C2 |
| RECOMBINANT PLASMID DNA OF pBiPr-ABIgA2m1F16-ht TO OBTAIN RECOMBINANT IMMUNOGLOBULIN A OF ISOTYPE IgA2m1 | 2016 |
|
RU2671477C2 |
| RECOMBINANT PLASMID DNA PBIPR-ABIGA1FI6-HT FOR OBTAINING RECOMBINANT IMMUNOGLOBULIN A IGOTH IGA1 | 2016 |
|
RU2656142C1 |
| METHOD FOR PRODUCING RECOMBINANT IGA2m1 ISOTYPE IMMUNOGLOBULIN IN MAMMALIAN CELLS | 2022 |
|
RU2801178C1 |
| RECOMBINED PLASMID DNA CODING CHIMERIC ANTIBODY AGAINST HUMAN TUMOUR NECROSIS FACTOR-ALPHA, EUKARYOTIC CELL LINE PRODUCING CHIMERIC ANTIBODY, AND METHOD OF CHIMERIC ANTIBODY OBTAINMENT | 2013 |
|
RU2555533C9 |
| RECOMBINANT PLASMID DNA, ENCODING HUMAN FACTOR VII PROTEIN SEQUENCE, CELL LINE BHK/F7, TRANSFORMED WITH PLASMID DNA | 2006 |
|
RU2337965C2 |
| PLASMID FOR EXPRESSION OF RECOMBINANT HUMAN CHORIONIC GONADOTROPIN (HCG), PLASMID FOR EXPRESSION OF RECOMBINANT ALPHA- AND BETA-SUBUNITS OF HCG, MONOCLONAL LINE OF MAMMALIAN CELLS - PRODUCER OF HCG, METHOD OF PRODUCING RECOMBINANT HCG | 2024 |
|
RU2834784C1 |
| PLASMID FOR EXPRESSION OF HUMAN RECOMBINANT BLOOD CLOTTING FACTOR IX, CHO CELL PRODUCING HUMAN RECOMBINANT BLOOD CLOTTING FACTOR IX AND METHOD OF PRODUCING SAID FACTOR | 2014 |
|
RU2585532C2 |
| RECOMBINANT PLASMID DNA OF pFUSE-MARX-29-PRAD-F2A/BChE-14 CONTAINING A MODIFIED HUMAN BUTYRYLCHOLINESTERASE GENE FOR EXPRESSION OF BUTYRYLCHOLINESTERASE GENE IN MAMMALIAN CELLS FOR THERAPY OF ORGANOPHOSPHORUS TOXIN POISONING | 2016 |
|
RU2688729C1 |
| METHOD OF PRODUCING DIMERIC FORM OF IMMUNOGLOBULIN IGA1-ISOTYPE IN MAMMALIAN CELLS | 2023 |
|
RU2822890C1 |
