METHOD FOR SIMULATING AN INTRAOCULAR INFECTION PROCESS Russian patent published in 2019 - IPC G09B23/28 A61F9/00 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to ophthalmology, and can be used for simulating intraocular infectious process. A sterile needle is inserted into an anterior eye chamber and an intraocular fluid is withdrawn in amount of 0.1 ml. Thereafter, the needle is left in the eye, the syringe is replaced and an equal volume of the strain suspension is introduced, wherein the infectious agent is Pseudomonas aeruginosa. Culture is pre-inoculated with a bacterial loop into test tubes with meat-peptone agar, cultivated in a thermostat at temperature of 37±2 °C in aerobic medium for 24 hours, then gently washed with physiological solution and brought concentration of microbial cells in 1 ml to 1.3 billion by Tarasevich's turbidity standard in a sterile test tube. Clinical signs of infection are observed 1–2 days after introduction of the strain.

EFFECT: method provides a technically simple and highly reproducible model of an intraocular infectious process with simultaneous control of the desired degree of severity of the injury as a result of the presence of time information after infection, when observing the first clinical signs of ophthalmopathology, a method for obtaining and concentration of microbial cells of Pseudomonas aeruginosa strain required for infection.

1 cl, 5 dwg, 3 ex