FIELD: measurement technology.
SUBSTANCE: invention relates to electrochemical analysis methods, particularly to analysis of solutions for determination of total antioxidant capacity. Invention relates to a method of determining antioxidant capacity of a solution using a potentiometric method, in which a phosphate buffer solution is prepared in advance, to which a system is added which contains an simultaneously oxidized and reduced form of metal in the complex compound K3[Fe(CN)6]/K4[Fe(CN)6], and evaluation of antioxidant capacity is carried out by change in oxidation-reduction potential of solution, measured between working platinum electrode and chloride-silver comparison electrode, recorded before and after introduction into initial solution of analyzed substance. From the total antioxidant capacity of the solution, a reducing and chelating reservoir is recovered, wherein the reducing capacity is pre-determined by potentiometric titration with an oxidized form of the reagent (K3[Fe(CN)6. Chelating reservoir is determined as the difference between the antioxidant capacity and the recovery capacity.
EFFECT: obtaining reliable quantitative information on restoring and chelating properties of analyzed objects with antioxidant action, as well as high accuracy and reliability of obtained results.
1 cl, 3 ex, 6 dwg
Title | Year | Author | Number |
---|---|---|---|
METHOD OF DETERMINING OXIDANT/ANTIOXIDANT ACTIVITY OF SUBSTANCES AND APPARATUS FOR REALISING SAID METHOD | 2012 |
|
RU2486499C1 |
METHOD OF POTENTIOMETRIC DETERMINATION OF ANTIOXIDANT/OXIDANT ACTIVITY WITH APPLICATION OF METAL COMPLEXES | 2013 |
|
RU2532406C1 |
METHOD OF NONINVASIVE POTENTIOMETRIC DETERMINATION OF OXIDANT/ANTIOXIDANT ACTIVITY OF BIOLOGICAL TISSUES AND DEVICE FOR ITS REALISATION | 2010 |
|
RU2433405C1 |
METHOD FOR DETERMINING ANTIOXIDANT CAPACITY OF SUBSTANCES | 2023 |
|
RU2825002C1 |
METHOD OF DETERMINATION OF OXIDANT/ANTI-OXIDANT ACTIVITY OF SOLUTIONS | 2002 |
|
RU2235998C2 |
METHOD OF DETERMINING INTEGRAL ANTIOXIDANT/OXIDANT ACTIVITY OF ORGANIC CONDENSED MEDIA | 2015 |
|
RU2595814C1 |
POTENTIOMETRIC METHOD FOR DETERMINING ANTIOXIDANT CAPACITY WITH 2,2'-DIPHENYL-1-PICRYLHYDRAZIL | 2022 |
|
RU2791901C1 |
COMPLEX FOR PREVENTION OF ORAL CAVITY OXIDATIVE STRESS | 2019 |
|
RU2748338C2 |
METHOD FOR DETERMINING ANTIOXIDANT ACTIVITY USING RADICAL INITIATORS | 2015 |
|
RU2618426C1 |
METHOD FOR DETERMINING ANTIOXIDANT ACTIVITY OF HERBAL RAW MATERIAL AND PHYTOPREPARATIONS BY DIFFERENTIAL SPECTROPHOTOMETRY | 2018 |
|
RU2712069C2 |
Authors
Dates
2020-01-17—Published
2019-03-13—Filed