FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, specifically to methods for cultivation of Chinese hamster ovary cells (CHO). Method comprises perfusion cultivation of CHO cell containing nucleic acid coding recombinant protein, under conditions sufficient for production of recombinant protein in a liquid medium containing poloxamer-188 in concentration 1.8 g/l or more 1.8 g/l.
EFFECT: invention widens the range of equipment.
22 cl, 5 ex, 1 tbl, 15 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| METHODS OF CULTIVATING MAMMAL CELLS | 2015 |
|
RU2811104C2 |
| METHODS FOR PREDICTION OF CELL VIABILITY | 2019 |
|
RU2786815C2 |
| METHOD OF CLONAL MICROREPRODUCTION OF GRAPES IN VITRO WITH DECONTAMINATION FROM MYCOPLASMAL INFECTION | 2013 |
|
RU2541769C1 |
| METHODS FOR CREATION OF CELLS BANKS WITH ULTRAHIGH DENSITY | 2015 |
|
RU2723129C2 |
| METHODS OF PERFUSION CULTIVATION AND USE THEREOF | 2015 |
|
RU2708919C2 |
| METHODS OF PERFUSION CULTIVATION AND USE THEREOF | 2015 |
|
RU2733539C2 |
| METHODS OF CREATING ULTRAHIGH DENSITY CELL BANKS | 2015 |
|
RU2826038C2 |
| METHOD OF CULTIVATION IN SYSTEM OF INOCULATING FERMENTORS (EMBODIMENTS) | 2015 |
|
RU2694327C2 |
| CULTIVATION PROCESSES IN SEED FERMENTER SYSTEM AND THEIR APPLICATION METHODS | 2015 |
|
RU2786997C2 |
| METHOD FOR PREPARING FED-BATCH ANTIBODY OR ITS FRAGMENT (VERSIONS) | 2007 |
|
RU2518289C2 |
