METHODS OF CREATING ULTRAHIGH DENSITY CELL BANKS Russian patent published in 2024 - IPC A01N1/02 C12M1/00 C12M3/06 

FIELD: biotechnology.

SUBSTANCE: invention relates to creation of blood banks. Method of obtaining a bank of frozen mammalian cells directly from a population of cultured cells involves culturing mammalian cells in a perfusion bioreactor at pH from 6.8 to 7.2, wherein the concentration of the population of cultured mammalian cells is from 2×10⁷ to 5×10⁷ cells/ml and supply of O₂ to maintain the value of dissolved oxygen (DO) ≥40% to obtain a cultured population of cells with ultrahigh density; adding a cryoprotectant to the cultured ultrahigh-density cell population; freezing at a controlled rate and obtaining a bank of frozen cells with ultrahigh density with a cell concentration of at least 1.0×10⁸ cells/ml.

EFFECT: proposed method of producing a bank of frozen mammalian cells enables to obtain a bank of frozen cells with a cell concentration of at least 1_0×10⁸ cells/ml without a high-quality cell concentration step and cell viability after defrosting of at least 95%.

12 cl, 24 dwg, 10 ex