FIELD: preservation of biological objects.
SUBSTANCE: invention refers to preservation of blood cells for creation of blood banks. Method for creation of banks of ultrahigh density frozen cells involves cultivation in perfusion conditions, which enables to obtain cell culture with ultrahigh density. Further, cell cultures are subjected to cryopreservation with the addition of a cryoprotector without the need for any cell concentration stages, wherein the bank of frozen cells with ultrahigh density is characterized by concentration of at least 1.0×108 cells. Cells are cultured in a bioreactor connected to a cell retention system.
EFFECT: disclosed method for producing a bank of frozen cells provides maintaining high cell viability and quality.
23 cl, 22 dwg, 10 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHODS OF CREATING ULTRAHIGH DENSITY CELL BANKS | 2015 |
|
RU2826038C2 |
| METHODS FOR CREATION OF HIGH DENSITY CELL BANK (VARIANTS) | 2014 |
|
RU2668796C2 |
| METHOD OF CULTIVATION IN SYSTEM OF INOCULATING FERMENTORS (EMBODIMENTS) | 2015 |
|
RU2694327C2 |
| CULTIVATION PROCESSES IN SEED FERMENTER SYSTEM AND THEIR APPLICATION METHODS | 2015 |
|
RU2786997C2 |
| PERFUSION FILTRATION METHOD FOR CONTINUOUS CULTURING OF CELL CULTURES | 2022 |
|
RU2794425C1 |
| SMALL-SCALE METHOD OF CULTIVATION OF SUSPENDED CELLS | 2016 |
|
RU2730656C2 |
| PERFUSION FILTRATION METHOD OF CONTINUOUS CULTURING OF CELL CULTURES | 2022 |
|
RU2800874C1 |
| METHODS OF PERFUSION CULTIVATION AND USE THEREOF | 2015 |
|
RU2708919C2 |
| METHODS OF PERFUSION CULTIVATION AND USE THEREOF | 2015 |
|
RU2733539C2 |
| PROCESS OF LARGE-SCALE PRODUCTION OF CAPPED AND UNCAPPED ANTIBODY CYSTEINES AND THEIR APPLICATION FOR THERAPEUTIC PROTEIN CONJUGATION | 2018 |
|
RU2759602C2 |
