FIELD: biotechnology.
SUBSTANCE: invention relates to new tools for CRISPR/Cas technology. The invention discloses a novel transcription activator comprising a complex of VP64 and a transcription activation site from an R-transactivator (RTA) with sequence length selected and optimized so that it can be assembled into an adeno-associated viral (AAV) vector while retaining its ability to activate the process of transcription of genetic information.
EFFECT: obtaining new transcription activator.
12 cl, 5 dwg, 4 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| GUIDED RNA TRANSCRIPTION REGULATION | 2014 |
|
RU2690935C2 |
| CRISPR/CAS9 TYPE II GENOME EDITING SYSTEM AND ITS USE | 2022 |
|
RU2794774C1 |
| TAU MODULATORS AND METHODS AND COMPOSITIONS FOR THEIR DELIVERY | 2017 |
|
RU2789459C2 |
| GENE EDITING USING MODIFIED CLOSED-END DNA (ceDNA) | 2018 |
|
RU2811724C2 |
| METHODS AND COMPOSITIONS FOR TREATING HUNTINGTON'S DISEASE | 2015 |
|
RU2691102C2 |
| METHODS AND COMPOSITIONS FOR TREATING HUNTINGTON'S DISEASE | 2014 |
|
RU2693891C1 |
| PaCas9 NUCLEASE | 2018 |
|
RU2706298C1 |
| ORTHOGONAL CAS9 PROTEINS FOR RNA-GUIDED GENE REGULATION AND EDITING | 2021 |
|
RU2771583C1 |
| ARTIFICIAL GENOME MODIFICATION FOR GENE EXPRESSION REGULATION | 2018 |
|
RU2767201C2 |
| ORTHOGONAL PROTEINS Cas9 FOR RNA-DIRECTED REGULATION AND EDITING OF GENES | 2014 |
|
RU2704981C2 |
