FIELD: chemistry.
SUBSTANCE: invention relates to a method of producing a poloxamer for use in a cell culture medium. Solid poloxamer is heated to 60-185 °C to form a liquid poloxamer. Liquid poloxamer is cooled to temperature below 50 °C to form solid thermally treated poloxamer. Cooling is not carried out in prilling or grinding device. Poloxamer contains copolymer of ethylene oxide and propylene oxide, where poloxamer has formula HO(C2H4O)n(C3H6O)m(C2H4O)nH, in which n equals 60–150 and m equals 25–60.
EFFECT: technical result is reduced variability of poloxamer and improved properties of poloxamer.
26 cl, 11 dwg, 17 tbl, 11 ex
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