CELL Russian patent published in 2020 - IPC C12N5/10 C12N5/783 A61K35/17 C07K19/00 

FIELD: immunology; cell biology.

SUBSTANCE: there are presented cytotoxic T-cell and natural killer cell (NK-cell) containing two chimeric antigenic receptors (CAR), one of which contains CD3ζ endodomain activating the cell in the presence of the first antigen, and the second one—tyrosine phosphatase CD148 or CD45 domain, which inhibits cell in the absence of a second antigen. Such a CAR system allows the cell to function according to the logical "AND" principle, i.e. the cell is activated only in the presence of both the first and second antigens. Additionally presented are: nucleic acid and sets of nucleic acids coding said CAR; vector and method of producing cytotoxic T-cell; as well as a pharmaceutical composition and a method of treating and/or preventing the disease by killing target tumor cells expressing a first antigen and a second antigen; use of the pharmaceutical composition and T-cell when preparing the medicinal agent and for treating and/or preventing the disease.

EFFECT: as result, T-cell and methods of its use for treating and/or preventing the disease are provided.

27 cl, 31 dwg, 6 tbl, 14 ex