CELL Russian patent published in 2020 - IPC C12N5/10 C12N5/783 A61K35/17 C07K19/00 

FIELD: immunology; cell biology.

SUBSTANCE: cytotoxic T-cell and a natural killer cell (NK-cell) containing two chimeric antigenic receptors (CAR), one of which contains zeta-endodomain CD3, activating the cell in the presence of the first antigen, and the second is the tyrosine phosphatase domain from the Src-containing homologue (SH2) of protein tyrosine phosphatase, which inhibits the cell in the presence of a second antigen. Such CAR system allows the cell to operate according to the principle of logical operator "AND-NOT", i.e. cell is activated in the presence of the first and absence of the second antigen. Additionally presented are: nucleic acid and sets of nucleic acids coding said CAR; vector and sets of vectors, as well as a method of producing a cytotoxic T-cell and a method of treating and/or preventing cancer by destroying tumor target cells expressing a first, but not a second antigen.

EFFECT: what is presented is a cytotoxic T-cell and a natural killer cell.

20 cl, 32 dwg, 8 tbl, 16 ex