FIELD: biotechnology.
SUBSTANCE: invention represents a method for surface sterilization of soya beans in vitro, including obtaining beans, their sterilization with an agent, washing, isolation of explants, cultivation on nutrient medium and recording. A distinctive feature of the method is that the beans are sterilized in a solution of the “AHD-2000 express” agent for 1.5 minutes, followed by 2-fold washing with sterile distilled water, wherein the agent solution is repeatedly used up to seven times.
EFFECT: proposed invention allows to increase yield of sterile and viable soya explants, to accelerate selection process in selection, to minimize harmful effect of sterilizing agent, which simplifies method of surface sterilization of soya beans in vitro.
1 cl, 8 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF OBTAINING RICE REGENERATES IN ANTHER CULTURE IN VITRO | 2018 |
|
RU2681339C1 |
| METHOD FOR MICROPROPAGATION OF BUCKWHEAT IN VITRO | 2022 |
|
RU2783357C1 |
| METHOD FOR PRODUCING NEW BUCKWHEAT GENOTYPES IN VITRO | 2022 |
|
RU2789885C1 |
| METHOD OF INCREASING FLAVONOID-FORMING ABILITY OF BUCKWHEAT TISSUE CULTURE IN VITRO | 2023 |
|
RU2811024C1 |
| NUTRIENT MEDIUM FOR MICROPROPAGATION OF BUCKWHEAT | 2022 |
|
RU2789883C1 |
| METHOD FOR HEALING POTATOES FROM VIRAL INFECTIONS IN VITRO | 2023 |
|
RU2805356C1 |
| PLANT PROTECTION AGENT AGAINST INFECTIOUS DISEASES OF CROPS | 2021 |
|
RU2767330C1 |
| METHOD OF MICROCLONAL PROPAGATION OF HONEYSUCKLE IN VITRO | 2023 |
|
RU2807118C1 |
| METHOD FOR PRESOWING SEED TREATMENT OF SPRING VETCH | 2021 |
|
RU2775314C1 |
| METHOD FOR INCREASING THE VIABILITY AND FERTILITY OF THE DROSOPHILA FRUIT FLY (DROSOPHILA MELANOGASTER) | 2021 |
|
RU2776012C1 |
