FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and represents a set of synthetic oligonucleotide primers for polymerase chain reaction (PCR), as well as synthetic oligonucleotide DNA probes for subsequent hybridisation. Invention can be used for DNA analysis for the probabilistic determination of a phenotype by the following criteria: AB0 blood group, Y-chromosome haplogroup, iris colour, hair colour, skin colour and gender. Invention also relates to a method for genotyping DNA markers associated with phenotypic characters having a value in practical forensics. Kit for genotyping contains a composition formed by pairs of primers for 63 loci in the human genome, which enables to perform multiplex PCR (sequence of primers is presented in SEQ ID NO: 1–SEQ ID NO: 107). Also, the composition for genotyping contains allele-specific oligonucleotide probes for hybridisation analysis (SEQ ID NO: 108–SEQ ID NO: 229). Primers are selected so that PCR-product has minimum length, which enables to successfully analyse degraded DNA samples.
EFFECT: composition of primers and oligonucleotide probes is suitable for different technological platforms, based on genotyping using DNA amplification with subsequent hybridisation.
1 cl, 3 ex, 2 tbl, 3 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF IDENTIFYING PERSON AND ESTABLISHING AFFINITY USING INDEL POLYMORPHISMS AND SET OF SYNTHETIC OLIGONUCLEOTIDES FOR GENOTYPING THEREOF | 2020 |
|
RU2738752C1 |
| METHOD FOR DETECTING GENETIC RISK FACTORS FOR DEVELOPMENT OF ALZHEIMER TYPE DEMENTIA ON THE BASIS OF HYDROGEL MATRIX BIOCHIP | 2022 |
|
RU2795795C1 |
| SET OF DIFFERENTIATING NUCLEOTIDES AND BIOCHIP APPLICABLE IN METHOD FOR GENETIC TYPING OF HUMAN Y-CHROMOSOMES HAPLOGROUP MARKERS: M130 (C), M145 (DE) | 2012 |
|
RU2539733C2 |
| DETERMINATION OF NUCLEIC ACIDS BY AMPLIFICATION BASED ON INCORPORATION INTO CHAIN | 2014 |
|
RU2694976C1 |
| NUCLEIC-ACID SEQUENCE FOR TRANSGENIC SOYBEAN TRANSFORMANT DBN9004 DETECTION IN A BIOLOGICAL SAMPLE, A KIT CONTAINING SUCH A SEQUENCE, AND A METHOD FOR SUCH DETECTION | 2017 |
|
RU2743397C2 |
| METHOD OF AMPLIFICATION AND IDENTIFICATION OF NUCLEIC ACIDS | 2019 |
|
RU2811465C2 |
| METHOD OF POLYMORPHIC MARKERS ANALYSIS IN THE GENES OF METABOLISM OF MEDICINAL PREPARATIONS AND THE GENES OF IMMUNE RESPONSE IN THE ACUTE LEUCOSISES THERAPY IN CHILDREN | 2016 |
|
RU2643333C1 |
| SET OF STR-MARKERS OF THE Y-CHROMOSOME FOR DETERMINING THE ETHNO-TERRITORIAL ORIGIN OF AN INDIVIDUAL BASED ON A SAMPLE OF HIS DNA | 2021 |
|
RU2804433C2 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENTLY MARKED PROBE IN TAQMAN FORMAT FOR DETECTION OF VIRUSES OF FLAVIVIRUS GENUS BY REAL-TIME PCR | 2022 |
|
RU2808520C1 |
| METHOD OF ANALYZING TERMINAL MUTATIONS IN BRCA1, BRCA2, ATM AND PALB2 GENES USING MULTIPLEX PCR AND SUBSEQUENT HYBRIDISATION WITH AN OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) | 2020 |
|
RU2729360C1 |
