FIELD: biochemistry.
SUBSTANCE: invention relates to a rodent for producing a variable domain of a human heavy chain, to a cell obtained therefrom, as well as to use of said rodent to obtain a nucleic acid sequence, encoding a human variable heavy chain domain, and use of said rodent to produce a human antibody. Also disclosed is a method of producing a nucleic acid sequence, which codes a human immunoglobulin heavy chain variable domain, comprising amplification of a nucleic acid, containing a rearranged human immunoglobulin heavy chain variable region gene from the above rodent lymphocyte or from a hybridoma derived from said lymphocyte. Invention also relates to a totipotent rodent cell for producing a rodent which produces a variable domain of the human heavy chain.
EFFECT: invention enables effective production of a variable domain of a human heavy chain.
25 cl, 22 dwg, 9 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| MICE EXPRESSING LIMITED REPERTOIRE OF IMMUNOGLOBULIN LIGHT CHAINS | 2014 |
|
RU2812814C2 |
| ADAM6 MICE | 2012 |
|
RU2722373C2 |
| NON-HUMAN ANIMALS WITH CONSTRUCTED LIGHT CHAIN OF LAMBDA IMMUNOGLOBULIN, AND THEIR USE | 2018 |
|
RU2778410C2 |
| MICE WITH HUMANIZED LIGHT CHAIN | 2012 |
|
RU2761639C2 |
| MICE WITH HUMANIZED UNIVERSAL LIGHT CHAIN | 2012 |
|
RU2787781C2 |
| ANIMALS OTHER THAN HUMANS WITH CONSTRUCTED LOCUS OF LIGHT LAMBDA CHAIN OF IMMUNOGLOBULIN | 2017 |
|
RU2757665C2 |
| GENETICALLY MODIFIED ANIMALS OTHER THAN HUMANS FOR PRODUCING THERAPEUTIC ANTIBODIES AGAINST PEPTIDE-MHC COMPLEXES, METHODS FOR PRODUCTION AND VERSIONS OF USE THEREOF | 2019 |
|
RU2819525C2 |
| ANTIBODIES TO CD38 AND COMBINATIONS WITH ANTIBODIES TO CD3 AND CD28 | 2018 |
|
RU2812910C2 |
| TRANSGENIC ANIMALS, OTHER THAN HUMANS, PRODUCING MODIFIED ANTIBODIES CONTAINING ONLY HEAVY CHAINS | 2017 |
|
RU2762835C2 |
| RESTRICTED IMMUNOGLOBULIN HEAVY CHAIN MICE | 2012 |
|
RU2603090C2 |
